

Dhyana 400D Cooled FSI sCMOS Camera, 2048 × 2048, 72% QE, USB 3.0
Dhyana 400DDesign Tools
A cooled monochrome sCMOS camera for routine fluorescence and luminescence imaging over USB 3.0.
The Dhyana 400D is an air-cooled, front-illuminated monochrome sCMOS camera built on the GSENSE2020 sensor, with 2048 × 2048 pixels of 6.5 µm. It pairs 72% peak QE with 2.1 e⁻ read noise in high-gain mode, and cooling to 35 °C below ambient holds dark current at 0.12 e⁻/pixel/s at −10 °C. It covers most fluorescence imaging tasks as well as bioluminescence and chemiluminescence work.
Key Features
- 72% peak QE at 595 nm and 2.1 e⁻ read noise (high gain)
- Air cooling to 35 °C below ambient; dark current 0.12 e⁻/pixel/s at −10 °C, reducing hot pixels in long exposures
- 6.5 µm pixels match the sampling needs of high-NA 40×, 60× and 100× microscope objectives
- 45 ke⁻ typical full well and 86.6 dB dynamic range
- 35 fps at 16 bit over USB 3.0, with hardware 2 × 2 binning and ROI
- SMA trigger I/O; supported in LabVIEW, MATLAB and Micro-Manager 2.0
Typical Applications
- Cell and tissue fluorescence imaging (bone, kidney, brain)
- Digital PCR (dPCR) readout
- Bioluminescence imaging
- Chemiluminescence imaging
- General fluorescence microscopy
Related Products
- Dhyana 201D — Compact FSI sCMOS Camera, 2048 × 1152, 6.5 µm Pixels, USB 3.0
- Dhyana 2100 — Global-Shutter CMOS Camera, 5120 × 4096, 450 fps
- Dhyana 400DC — Cooled Color sCMOS Camera, 2048 × 2048, 16-bit, USB 3.0
Browse all: sCMOS Cameras · Cameras
Model: Dhyana 400D · Acquisition software and drivers are supplied with the camera. Contact WaveQuanta for a quote and lead time.
| Sensor Type | FSI sCMOS |
| Sensor | GSENSE2020 |
| Color / Mono | Mono |
| Peak QE | 72% @ 595 nm |
| Resolution | 2048 × 2048 |
| Pixel Size | 6.5 µm × 6.5 µm |
| Effective Area | 13.3 mm × 13.3 mm |
| Array Diagonal | 18.8 mm |
| Full-Well Capacity | 45 ke⁻ (typical) |
| Readout Noise | 2.1 e⁻ (high gain) |
| Dynamic Range | 86.6 dB (typical) |
| Frame Rate | 35 fps @ 16 bit |
| Shutter Type | Rolling |
| Exposure Time | 13 µs – 10 s |
| Binning | 2 × 2 |
| ROI | Supported |
| Cooling Method | Air |
| Cooling Temperature | 35 °C below ambient |
| Dark Current | 0.12 e⁻/pixel/s @ −10 °C |
| Bit Depth | 12 bit, 16 bit |
| Data Interface | USB 3.0 |
| Trigger | Hardware, software; SMA connectors |
| Trigger Output | Exposure Start, Global Exposure, Readout End |
| Optical Interface | C-mount |
| Power Supply | 12 V / 8 A |
| Power Consumption | 60 W |
| Dimensions | 120 mm × 119 mm × 121 mm (H × W × L) |
| Weight | 1853 g |
| Operating Environment | Working 0–40 °C, 10–85% RH |
| Operating System | Windows, Linux |
| Software / SDK Support | Acquisition and analysis software, LabVIEW, MATLAB, Micro-Manager 2.0; C/C++/C#/Python SDK |






